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Vitamin C also plays an important role in supporting the bodys immune system, wound healing, and connective tissue synthesis

Trends Biochem Sci 30:325333 Flentjar NJ, Crack PJ, Boyd R, Malin M, de Haan JB, Hertzog P, Kola I, Iannello R (2002) Mice lacking glutathione peroxidase-1 activity show increased tunel staining and an accelerated inflammatory response in brain following a cold-induced injury

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Any insoluble material was pelleted by centrifugation (13,000 g , 10 min, 4 C) and then 25 l of the supernatant CL was transferred to a clean microcentrifuge tube